Analysis of Collagen Architecture Alterations in Human Ovarian Cancer Via SHG Polarization and Texture Analyses

時間地點:10:30 am, Dec 05 (Wed), 2018; R1-B1122 Conference Room

研討講者:Paul J. Campagnola Ph.D

November 16, 2018

Abstract

Remodeling of the extracellular matrix in human ovarian cancer can be reflected in increased collagen concentration, changes in alignment within fibrils and fibers and/or up-regulation of different collagen isoforms. We used pixel-based SHG polarization analyses to discriminate ex vivo human tissues (normal stroma, benign tumors, and high grade serous tumors) by: i) determination of i) helical pitch angle via the single axis molecular model, ii) dipole alignment within fibrils via anisotropy, and iii) chirality via SHG circular dichroism (SHG-CD). The largest differences were between normal stroma and benign tumors, consistent with gene expression showing Col III is up-regulated in the latter. The different tissues also displayed differing SHG anisotropies and SHG-CD responses, consistent with either Col III incorporation or randomization of Col I alignment within benign and high-grade tumors fibrils. These results collectively indicate the fibril assemblies are distinct in all tissues and likely result from synthesis of new collagen rather than remodeling of existing collagen. We also implemented a form of 3D texture analysis to delineate the fibrillar morphology observed in SHG images of normal stroma and a spectrum of ovarian benign and malignant tumors (6 classes). We developed a tailored set of 3D filters which extract textural features in the 3D image sets to build statistical models of each class.  By applying k-nearest neighbor classification using these models, we achieved 83-91% accuracies for the six classes. This classification based on ECM structural changes will complement conventional classification based on genetic profiles and can serve as an additional biomarker.


Paul J. Campagnola Ph.D

Professor, Department of Biomed Eng & Associate Chair, Graduate Advising Department of Medical Physics,

University of Wisconsin Madison



Dr. Paul J. Campagnola currently is a Full Professor at the Department of Biomedical Engineering and is also an Associate Chair for Graduate Advising at the Department of Medical Physics at the University of Wisconsin, Madison. After receiving his PhD from Yale, Dr. Campagnola went on to University of Colorado and obtained his postdoctoral training in the Department of Chemistry. Through his career, Dr. Campagnola has made significant contributions in microscopy, micro/nanofabrication and bio-imaging, which has successfully combined microscopic imaging techniques to address problems in life science. Dr. Campagnola and his lab currently focus on studying alterations of the extracellular matrix (ECM) in cancer as well as in connective tissue disorders. They use Second Harmonic Generation imaging microscopy for imaging structural aspects of tissues, where the approaches will ultimately be implemented as clinical diagnostics. They also use 3D micro/nanofabrication approaches to create biomimetic models of the ECM to study signaling pathways associated with cancer, and also to provide insight into the design of tissue engineering scaffolds. Dr. Campagnola’s impressive publications include those published in top journals such as Nature, J. Chem. Phys., Optics Express and ACS Nano.