Single-particle measurement and quality control technology for clinical application of exosomes

時間地點：02:30 pm, Jul 26 (Fri), 2024; R1-1042 Conference Room

研討講者：Takanori Ichiki, PhD

July 10, 2024

Absract

    In recent times, there has been a surge in expectations regarding the development of novel diagnostic and therapeutic technologies employing extracellular vesicles (EVs), including exosomes. However, it remains a challenge to measure and manipulate heterogeneous particle populations with diameters spanning from several tens to 100 nm. Consequently, there is a pressing need to establish a robust foundation for evaluation technology that can ensure the reliability, safety, and standardization of exosome-based medicine.

Amidst these challenges, the technique of nanoparticle measurement using scattering imaging, known as Nanoparticle Tracking Analysis (NTA), is regaining attention as a single-particle analysis method for assessing the concentration and size of EVs. Our team has pioneered a nanoparticle measurement system that integrates microfluidic devices and scattering imaging. Additionally, we have implemented deep learning techniques to enhance the precision and sophistication of the NTA method.

Keywords: exosomes, nanoparticle tracking analysis, deep learning



REFERENCES

[1] T. Akagi, K. Kato, M. Kobayashi, N. Kosaka, T. Ochiya, and T. Ichiki, “On-chip immunoelectrophoresis of extracellular vesicles released from human breast cancer cells”, PLOS ONE 10(4), e0123603 (2015).

[2] T. Akagi and T. Ichiki, “Microcapillary chip-based extracellular vesicle profiling system”, in W. P. Kuo and S. Jia, edit. Extracellular vesicles: Methods and protocols, p. 209–17, Springer New York (2017).

[3] H. Fukuda, H. Kuramochi, Y. Shibuta, and T. Ichiki, “Analysis of Brownian motion trajectories of non-spherical nanoparticles using deep learning", APL Machine Learning 1, 046104 (2023).

Takanori Ichiki, PhD

Dept. of Materials Engineering/Bioengineering

University of Tokyo

Takanori Ichiki, PhD

AFFILIATION AND ADDRESS: 

Dept. of Materials Engineering/Dept. of Bioengineering

School of Engineering, The University of Tokyo

7-3-1 Hongo, Bunkyo-ku, Japan

TELEPHONE No:  +81-3-5841-1180

FAX No:  +81-3-5841-1180

E-MAIL: ichiki@bionano.t.u-tokyo.ac.jp

Website: https://bionano.t.u-tokyo.ac.jp/en/

https://iconm.kawasaki-net.ne.jp/en/index.html

https://change.kawasaki-net.ne.jp/en/

CAREER HISTORY:

Takanori Ichiki obtained his M.S. and Ph.D. degrees in Metallurgy from the University of Tokyo in 1992 and 1995, respectively. He joined the Department of Electric and Electronics Engineering, Toyo University in 1995, where he studied ULSI nano/microfabrication technology and extended it to the nanobiodevice. In 2004, he was assigned to the University of Tokyo, as Associate Professor of Department of Bioengineering and Institute of Engineering Innovation from 2006 to 2015. In 2016 he was appointed Professor of Department of Materials Engineering. He is also the Research Director of Innovation Center of NanoMedicine (iCONM), Institute of Industry Promotion-Kawasaki, that launched in 2015, and founded a venture company, iXstream Inc. in 2018. He has been the project leader of MEXT/JST “Program on open innovation platform for industry-academia co-creation (COI-NEXT)” from 2022. He is a fellow of the Japan Society of Applied Physics and a board member of the Japanese Society of Extracellular Vesicles.